Transcript Nakano-20040615-5.ppt

Trends of PCB isomeric patterns
in Japanese environmental media,
food, breast milk and blood
in view of risk assessment
Takeshi Nakano1, Yoshimasa Konishi2,
Rie Masho3 and Chiharu Tohyama4
２,３,７,８－TCDD
７５ congeners
７５ congeners
１,２,３,５,６,７－HxCN
２,３,４,７,８－PeCDF
１３５congeners
２０９ congeners
３,４,５,３’,４’－Co-PCB
Trend of PCB Use in Japan
Total 58,000t
12000
10000
8000
使用量(t)
Amount
of PCB use (t)
6000
4000
2000
54
55
56
57
58
59
1960
61
62
63
64
1965
66
67
68
69
1970
71
72
0
year
PCB product use in Japan
(t)
製品別使用量(1969-1971)
1969-1971
15,000
10,000
5,000
0
KC300
KC400
KC-300 400
Ar-1242 1248
KC500
KC600
500 600
1254 1260
D10CB
N9CBs
30
KC600
KC500
10
0
KC600
製品別使用量(1969-1971)
50
5
40
0
KC500
25
10
0
Japan
D10CB
15,000
N9CBs
0
KC400
O8CBs
KC300
H7CBs
KC400
H6CBs
50
P5CBs
10
T4CBs
D10CB
N9CBs
O8CBs
(t)
T3CBs
D10CB
N9CBs
O8CBs
H7CBs
H6CBs
P5CBs
T4CBs
T3CBs
D2CBs
M1CBs
KC300
D2CBs
MCBｓ
D10CB
N9CBs
20
O8CBs
30
O8CBs
H7CBs
H6CBs
P5CBs
T4CBs
T3CBs
D2CBs
M1CBs
30
H7CBs
H6CBs
P5CBs
T4CBs
T3CBs
D2CBs
M1CBs
30
H7CBs
H6CBs
P5CBs
T4CBs
T3CBs
20
D2CBs
M1CBs
50
KC300
40
20
Japan
0
10,000
5,000
0
40
KC600
KC400
20
10
Homologue pattern
reflects product use
60
50
40
KC500
30
20
日本
15
10
PCB
Homologue Distribution
and
Source Estimation
30
25
20
15
Insulator
10
5
0
capacitor
45
40
KC６００
Ship paint
5
0
D10CBs
10
D10CBs
15
N9CBs
20
N9CBs
25
O8CBs
30
O8CBs
35
H7CBs
KC600
H7CBs
H6CBs
P5CBs
D10CBs
N9CBs
O8CBs
H7CBs
H6CBs
P5CBs
T4CBs
T3CBs
KC５００
50
45
40
35
30
25
20
15
10
5
0
H6CBs
KC４００
D2CBs
Source estimation
P5CBs
0
T4CBs
Sediment A
T4CBs
40
T3CBs
底質(KC300由来)
45
40
35
30
25
20
15
10
5
0
T3CBs
60
capacitor
D2CBs
20
M1CBs
KC３００
M1CBs
40
D2CBs
35
N.C.P.
D10CBs
50
M1CBs
KC400
D10CBs
KC300
D10CBs
10
N9CBs
0
N9CBs
20
O8CBs
H7CBs
H6CBs
10
N9CBs
30
O8CBs
H7CBs
H6CBs
P5CBs
T4CBs
T3CBs
D2CBs
M1CBs
30
O8CBs
H7CBs
45
40
P5CBs
T4CBs
T3CBs
D2CBs
M1CBs
50
H6CBs
50
P5CBs
T4CBs
T3CBs
D2CBs
M1CBs
60
KC500
Insulator
底質(KC600由来)
Sediment B
To evaluate environmental risk
Daily variation of PCDD/Fs
and PCBs in ambient air
20
15
8-1
8-2
8-3
8-4
8-5
8-6
8-7
8-8
8-9
8-10
8-11
8-12
8-14
8-15
8-16
8-17
8-18
8-20
8-21
8-22
8-23
8-24
8-25
8-26
8-27
8-28
8-30
8-31
3
concentration (pg/m )
Daily variation of Dioxin Concentration
50
45
Hi-vol.
40
PCDF
PCDD
35
30
25
Low-vol.
（PCDD+PCDF)
10
5
0
Time trend of PCDD/F levels
in ambient air
Time trend of PCDDs/PCDFs（TEQ）
1.2
PCDDs/DFs
TEQ
1.0
0.8
0.6
0.4
0.2
Matsumura et al, symposium of J.Env.Chem, 2003.6
2002
2001
2000
1999
1998
1997
1996
1995
1994
1993
1992
1991
1990
1989
1988
1987
1986
1985
1984
1983
1982
1981
0.0
1980
PCDDs/PCDFs TEQ (pg-TEQ/m 3 )
Dioxin Emission Reduction Plan
0.50
1997
0.40
1998
0.30
1999
0.20
2000
0.10
2001
Emission inventry (g-TEQ/year）
Matsumura et al, symposium of J.Env.Chem, 2003.6
0
1000
2000
3000
4000
5000
6000
7000
0.00
8000
PCDDs/PCDFs（pg-TEQ/m3)
Emission inventry vs Level in air
Matsumura et al, symposium of J.Env.Chem, 2003.6
2002
2001
2000
1999
1998
1997
1996
1995
0.06
1994
1993
1992
1991
1990
1989
1988
1987
1986
1985
1984
1983
1982
1981
1980
Co-PCBs TEQ (pg-TEQ/m 3 )
Time trend of Co-PCBs（TEQ）
0.07
Co-PCBs
TEQ
0.05
0.04
0.03
0.02
0.01
0.00
2002
2001
2000
1999
1998
1997
1996
1995
0.06
1994
1993
1992
1991
1990
1989
1988
1987
1986
1985
1984
1983
1982
1981
1980
Co-PCBs TEQ (pg-TEQ/m 3 )
10000
1954
1955
1956
1957
1958
1959
1960
1961
1962
1963
1964
1965
1966
1967
1968
1969
1970
1971
1972
1973
1974
1975
1976
1977
1978
1979
1980
1981
1982
1983
1984
1985
1986
1987
1988
1989
1990
1991
1992
1993
1994
1995
1996
1997
1998
1999
2000
2001
2002
Time trend of Co-PCBs（TEQ）
12000
PCB (t)
8000
6000
4000
2000
0
year
0.07
Co-PCBs
TEQ
0.05
0.04
0.03
0.02
0.01
0.00
Time trend of PCB levels
in ambient air
(log scale)
33
(ng/m
(pg/m ))
1000
Ambient air
100
10
1
0.1
0.01
1970
1975
1980
1985
1990
1995
2000
2005
33
Time
trend
of
PCBs
concentration
in
ambient
air
(ng/m
Time trend of PCBs concentration in ambient air (pg/m ))
Time trend of PCB levels
in
breast milk
Time trend of breast milk levels
µg / g-fat
µg g-fat 0.3
0.2
PCB
0.1
0
pg TEQ/g-fat 6060
pg TEQ/g-fat
40
40
Dioxins
20
20
0
0
Konishi
etetal.al (2003)
(2003)Organohalogen compds
Konishi
Year
Year(1973-2000)
(1973-2000)
Development of congener specific
analysis for PCBs using HT8-PCB
Congener
mono
di
tri
tetra
penta
hexa
hepta
octa
nona
deca
total
number
of isomer
3
12
24
42
46
42
24
12
3
1
209
number
of peak
3
10
23
39
38
40
23
12
3
1
192
GC/MS-SIM chromatogram of TrCB (HT8-PCB)
Intensity
T3CB
PCB product
4000000
0
12
13
14
15
16
17
18
Retention Time (min)
19
20
21
22
23
#37
#35
#38
#39
#36
#22
#21
#20
#33
#26
#25
#31
#28
#23
#34
#29
#32
#16
#18
#17
#24
#27
#30
#19
Calculated Retention Time
Intensity
13C-T3CB
13C-TrCB
0
12
13
14
15
16
17
18
Retention Time (min)
19
Matsumura et al, J. Env. Chem. (2002)
20
21
22
23
GC/MS-SIM chromatogram of PeCB (HT8-PCB)
Intensity
P5CB
800000
PCB product
400000
0
20
22
24
26
28
30
Retention Time (min)
32
34
36
38
#104
#96
#103
#100
#94
#102
#93
#98
#95
#88
#91
#121
#92
#84
#89
#90
#101
#113
#99
#112
#119
#83
#108
#86
#117
#97
#125
#116
#87
#115
#111
#85
#120
#110
#82
#124
#109
#107
#123
#106
#118
#114
#122
#105
#127
#126
Calculated Retention Time
Intensity
13C-P5CB
50000
13C-PeCB
0
20
22
24
26
28
30
Retention Time (min)
32
Matsumura et al, J. Env. Chem. (2002)
34
36
38
Congener profiles of PCBs
in environmental samples
50
KC300-600
40
30
20
10
0
#11 (3,3’)
60
Sediment
50
#35 (3,3’,4)
40
30
20
10
0
50
Water
40
30
20
10
0
80
70
60
50
40
30
20
10
0
Congener profiles of PCBs(Di-, Tri-) in environmental samples
#37
#35
#33/#20
#31/#28
#25
#26
#16/#32
#18/#17
#15
#12/#13
#11
#8/#5
Air (gas phase)
#4
40
35
30
25
20
15
10
5
0
Rain
Congener profiles of PCBs
in environmental
and
human samples
Isomer
Isomerdistribution
distribution（HxCB）
（HxCB）
PCB
PCBproduct
product
Intensity
HW1013+KC3-600
124328
0
18
19
20
21
22
Retention Time (min)
23
24
sediment
sediment
Intensity
01Sed-55
32392
0
18
19
20
21
22
23
24
Retention Time (min)
sea
sea water
water
Intensity
Portisland7/19-6L
5128
992
18
19
20
21
22
23
human
humanmilk
milk
Intensity
MM-44
47792
0
18
19
20
21
22
23
24
adipose
adiposetissue
tissue
o-neck-fat
162432
Intensity
24
0
18
19
20
#153
#153(2,2',4,4',5,5'-)
(2,2',4,4',5,5'-)
21
22
23
24
#138(2,2',3,4,4',5'-)
#138(2,2',3,4,4',5'-)
Congener profiles of PCBs(Hexa-) in environmental and human samples
Isomer
Isomerdistribution
distribution（HpCB）
（HpCB）
PCB
PCBproduct
product
Intensity
HW1013+KC3-600
74056
0
20.0
20.5
21.0
21.5
22.0
22.5
23.0
23.5
Retention Time (min)
24.0
24.5
25.0 25.5
sediment
sediment
Intensity
01Sed-55
8744
960
20.0
20.5
21.0
21.5
22.0
22.5
23.0
23.5
24.0
24.5
25.0 25.5
Retention Time (min)
sea
sea water
water
Intensity
Portisland7/19-6L
2080
984
20.0
20.5
21.0
21.5
22.0
22.5
23.0
23.5
24.0
24.5
human
humanmilk
milk
MM-44
Intensity
25.0 25.5
16312
944
20.0
20.5
21.0
21.5
22.0
22.5
23.0
23.5
24.0
24.5
25.0 25.5
Retention Time (min)
adipose
adiposetissue
tissue
Intensity
o-neck-fat
42888
0
20.0
20.5
21.0
21.5
#187
#187
22.0
22.5
23.0
23.5
Retention Time (min)
#180
#180
24.0
24.5
25.0 25.5
#170
#170
Congener profiles of PCBs(Hepta-) in environmental and human samples
Congener profiles of PCBs
in human samples
(breast milk)
#54
#50
#53
#51
#45
#46
#52, #69
#73
#43
#49
#65, #75
#48, #47
#62
#44
#59
#42
#64
#72
#71
#41
#68
#40
#57
#67
#63
#58
#61
#74
#70
#76
#80
#66
#55
#60
#56
#79
#78
#81
#77
14
10
50
45
#54
#50
#53
#51
#45
#46
#52, #69
#73
#43
#49
#65, #75
#48, #47
#62
#44
#59
#42
#64
#72
#71
#41
#68
#40
#57
#67
#63
#58
#61
#74
#70
#76
#80
#66
#55
#60
#56
#79
#78
#81
#77
16
T4CBs
25-25
#52
PCB product
12
25-23
#44
8
T4CBs
mother milk
40
30
25
15
10
Isomers
Isomer profiles of TeCB
#70
KC3-600/MBP-CG
#66
25-34
245-4
#74
23-34
#56
6
4
#60
2
0
Isomers
245-4
#74
MM44
35
24-34
#66
20
234-4
#60
5
0
14
10
8
0
Isomers
50
40
35
30
#104
#96
#103
#100
#94
#102, #93
#98, #95
#88
#91
#121
#92
#84
#89
#90
#101
#113
#99
#112, #119
#83
#115
#86, #117, #97
#125, #116
#87, #108
#111
#85
#120, #110
#82
#124
#109, #107
#123
#106
#118
#114
#122
#105
#127
#126
16
#104
#96
#103
#100
#94
#102, #93
#98, #95
#88
#91
#121
#92
#84
#89
#90
#101
#113
#99
#112, #119
#83
#115
#86, #117, #97
#125, #116
#87, #108
#111
#85
#120, #110
#82
#124
#109, #107
#123
#106
#118
#114
#122
#105
#127
#126
18
236-25
#95
245-25
#101
P5CBs
PCB product
mother
milk
P5CBs
45
245-24
#99
25
20
Isomers
Isomer profiles of PeCB
236-34
KC3-600/MBP-CG
#110
#118
12
245-24
#99
#105
6
4
2
245-34
#118
MM44
234-34
#105
15
10
5
0
0
Isomers
50
45
0
#155
#150
#152
#145
#136
#148
#154
#151
#135
#144
#147
#149
#139
#140
#143
#134
#142
#131
#133
#165
#146
#132
#161
#153
#168
#141
#137
#130
#164, #163
#138
#160
#158
#129
#166
#159
#128
#162
#167
#156
#157
#169
20
#155
#150
#152
#145
#136
#148
#154
#151
#135
#144
#147
#149
#139
#140
#143
#134
#142
#131
#133
#165
#146
#132
#161
#153
#168
#141
#137
#130
#164, #163
#138
#160
#158
#129
#166
#159
#128
#162
#167
#156
#157
#169
25
H6CBs
236-245 PCB product
#149
15
H6CBs
mother milk
40
#153
245-245
234-245
#138
#153
245-245
35
30
25
Isomers
Isomer profiles of HxCB
KC3-600/MBP-CG
10
5
MM44
234-245
#138
20
15
10
5
Isomers
Isomer profiles of HpCB
#180
245-2345
#189
15
#190
245-2356
#187
#170
#189
#190
#170
15
#191
#193
245-2356
#187
#191
35
#180
PCB product
#180
#193
breast milk
#192
#172
#173
#171
#177
#181
H7CBs
#180
H7CBs
#192
#172
#173
#171
#177
#181
45
#174
#185
#183
25
#174
40
#185
#183
20
#182, #187
0
#182, #187
25
#175
#178
#186
#176
#179
#184
#188
20
#175
#178
#186
#176
#179
#184
#188
30
KC3-600/MBP-CG
245-2345
234-2345
#170
10
5
Isomers
MM44
30
234-2345
#170
10
5
0
Congener profiles of PCBs
in human samples
(pooled blood)
#28
＃28
Tri-CBs
24-4
＃31
Tetra-CBs
245-4
＃74
#74
＃80
Penta-CBs
#99
#118
245-34 ＃118
＃99
245-24
Hexa-CBs
＃153
245-234
#153
245-245
＃163/
＃164
Hepta-CBs
#187
＃187
245-2356
#138
＃138
245-2345
＃180
#180
＃170
Chromatogram of PCB in human blood （3~7Cl）
Hirai et al, (2003) Organohalogen compounds
Levels of the predominant PCB congeners in human blood
(n=24)
Congeners Mean± 2S.D. Existence Ratio*
(BZ#)
245-245
245-2345
245-234
245-2356
245-34
245-24
245-4
245-235
2345-234
2345-34
#153
#180
#138
#187
#118
#163,164
#99
#74
#146
#170
#156
Sum
(ng /g-lipid)
28.0± 28.0
14.9± 16.6
10.4± 9.2
8.3± 8.5
6.9± 5.9
6.4± 6.2
4.7± 4.2
4.6± 2.6
4.1± 3.7
3.8± 4.1
2.8± 3.0
(%)
22.3
11.8
8.3
6.6
5.5
5.1
3.8
3.6
3.3
3.0
2.3
75.6
* Mean is compare to total PCBs level
Hirai et al, (2003) Organohalogen compounds
cumulative
(%)
22.3
34.1
42.4
49.0
54.5
59.6
63.4
67.0
70.3
73.3
75.6
human milk/adipose tissue
#74
(2,4,4',5-)
#99
(2,2’,4,4',5-)
#118
(2,3’,4,4',5-)
#153
(2,2',4,4',5,5'-)
#74 (4-245)
#153 (245-245)
#138
(2,2',3,4,4',5'-)
#99 (24-245)
#138 (234-245)
#187
(2,2',3,4’,5,5’,6-)
#118 (34-245)
#187 (2356-245)
PCB congeners predominant in human samples
PCB levels
and
Homologue profiles
in
Environmental samples
0.1-10ng/m3
1
Water
W a te r
A tm o s p h e re
0 .0 1
0 .0 1
0 .1
75%
M e d ia n
1000
0.05-800ng/g-dry
h ep tach lo r
DDTs
d ieldrin
10
M in
ch lo rd an e
100
25%
PCBs
10000
S e d im e n t
Sediment
HCB
M ax
HCHs
100000
ald rin
HCHs
HCB
h eptach lo r
C o n c e n tra tio n (p g /ｇ )
M in
PCBs
ch lo rd an e
1
en drin
25%
100
10
d ield rin
75%
DDTs
1000
M ax
M e d ia n
ald rin
C o n c e n tra tio n (p g /ｇ -ｄ ｒｙ )
100000
en d rin
1000000
1000000
10000
HCHs
HCB
M in
PCBs
10
25%
h eptach lor
75%
aldrin
100
en d rin
M e d ia n
0 .1
Air
DDTs
M ax
1000
d ieldrin
0 .1
10000
ch lo rd an e
1
C o n c e n tra tio n (p g / ｌ )
ch lo rd an e
M in
HCHs
h ep tach lor
HCB
10
0.1-10ng/L
100000
PCBs
25%
en drin
100
d ieldrin
75%
M e d ia n
DDTs
M ax
1000
ald rin
C o n c e n tra tio n (p g /m 3 )
10000
B io t a
1
0.1-800ng/g-wet
Biota
PCB levels in air, water, sediment and
biota (2002, MOE Japan)
Homologue profiles of PCBs
in human samples
30
20
10
0
DeCB
Bir d
NoCB
50
OcCB
ng/g-lipid
HpCB
0
HxCB
Water
PeCB
40
TeCB
0
abundance(%)
10
TrCB
NoCBs
OcCBs
20
DiCB
NoCBs
OcCBs
HpCBs
HxCBs
PeCBs
TeCBs
TrCBs
Air
MCB
NoCBs
OcCBs
30
HpCBs
HxCBs
PeCBs
TeCBs
TrCBs
DiCBs
MoCBs
30
HpCBs
HxCBs
PeCBs
TeCBs
Bird
TrCBs
40
DiCBs
Water
DiCBs
MoCBs
Air
MoCBs
40
50
45
40
35
30
25
20
15
10
5
0
Human blood
20
10
Breast milk
70
60
50
40
30
20
10
0
Congener profiles of TrCB,TeCB
in human
and
combustion sample (A)
_19
_30
_18
_17
_32
_16
_31
_28
_22
_35
_37
_53
_51
_45
_52_69
_43_49
_48_47
_44
_64
_74
_70
_66
_79
_78
_81
_77
_19
_30
_18
_17
_32
_16
_31
_28
_22
_35
_37
_53
_51
_45
_52_69
_43_49
_48_47
_44
_64
_74
_70
_66
_79
_78
_81
_77
_19
_30
_18
_17
_32
_16
_31
_28
_22
_35
_37
_53
_51
_45
_52_69
_43_49
_48_47
_44
_64
_74
_70
_66
_79
_78
_81
_77
8
6
KC400
70
60
50
40
30
20
10
0
4
3
PCB product
4
2
0
PCB product
医療廃棄物
Combustion
Combustion
5
母乳
Breast milk
2
1
0
Breast milk
PCB congeners predominant in human samples (TrCB, TeCB)
Congener profiles of TrCB,TeCB
in combustion sample (B)
(stack gas)
Compound View
Page 1
DqData : C:\Documents and Settings\hyogo\My Documents\0404PCB•ˆ—•ƒf•[ƒ^•ˆ—•\DiokData\PCBN38Along-new\0404PCBN38Along-003
Injection : PCBGas-GeomeltTO-Exhaust
T3CB / Average
129564
#47
TrCB
Intensity
24-24
0
12
14
16
18
#51
T4CB / Average
172261
20
22
Retention Time (min)
24
#68
26
30
32
TeCB
24-35
Intensity
24-26
28
0
12
14
16
18
20
22
Retention Time (min)
24
26
28
30
32
Congener profiles of PCB
in human sample
Contribution of DL-PCBs in breast milk
#196, #203
#199
#170
#180
#177
#183
#187
#186, #178
#156
#167
#138
#153
#146
#105
40
#118
#99
#101, #90
#66
#74
189
180
170
169
167
157
156
126
123
118
114
105
81
77
15
ng/g-lipid
12 DL-PCB(TEF)+di-ortho-PCB
10
5
0
ng/g-lipid
Predominant PCB in product
30
20
10
0
Congener profiles of PCB
in food sample
ng/day
500
1982
10-12_1982
400
300
200
100
0
31_28
52
66
74
99
101
110
118
138
149
153
180
187
74
99
101
110
118
138
149
153
180
187
ng/day
500
2001
10-12_2001
400
300
200
100
0
31_28
52
66
PCB congener profiles in food sample (Konishi et al )
Congener profiles of PCB
in human sample
#153
#138
#118
#74
#180
#99
1973
#187
PCB congener composition in human breast milk
#153
2000
#74
#99
#118
#138
#187
#180
PCB congener composition in human breast milk
#153
Salmon
#101
#138
#118
#66
#187
#180
PCB congener composition in salmon
#153
Breast milk
#74
#99
#118
#138
#187
#180
PCB congener composition in human breast milk
PCB product
human
#74 < #66
245-4
24-34
#74 > #66
245-4
24-34
#99 < #101
245-24 245-25
#99 > #101
245-24 245-25
#146 < #149
245-235 245-236
#146 > #149
245-235 245-236
PCB metabolite
OH-PCB, (OH)2-PCB
4-OH-3,3’,4’,5-tetrachlorobiphenyl
Thyroxine
4-OH-CB-79
Thyroxine & TTR binding OH-PCB
4-OH-2,3,3’,4’,5-pentachlorobiphenyl
Thyroxine
4-OH-CB-107
Thyroxine & TTR binding OH-PCB
Cl
Cl
CB-105
Cl
Cl
Cl
Cl
Cl
Cl
Cl
Cl
Cl
Cl
OH
Cl
4-OH-CB-107
Cl
Cl
CB-118
Cl
CB-138
Cl
Cl
Cl
Cl
Cl
Cl
Cl
Cl
Cl
Cl
Cl
Cl
Cl
OH
Cl
Cl
4-OH-CB-146
CB-153
Cl
Cl
Cl
CB-183
Cl
Cl
Cl
Cl
Cl
Cl
Cl
Cl
Cl
Cl
Cl
Cl
Cl
Cl
Cl
OH
Cl
Cl
Cl
Cl
Cl
CB-187
4-OH-CB-187
Fish (Sea Bass)
OH-TrCB
OH-TeCB
4-OH-CB-107 ?
OH-TrCB
OH-PeCB
Sediment
OH-TeCB
OH-PeCB
Chromatogram of PCB-OH by methylation
(Sakiyama et al. J.Env.Chem.)
PCB metabolite
MeSO2-PCB
Cl
Cl
CB-
Cl
Cl
Cl
SO2CH3
4-MeSO2-CB-87
CB-
CBCl
Cl
Cl
Cl
Cl
SO2CH3
4-MeSO2-CB-101
CB-
Cl
Cl
CBCl
Cl
Cl
Cl
SO2CH3
4-MeSO2-CB-149
CB-
Chiral metabolite
4-MeSO2-CB-149
Chiral metabolite
Proposal of index
PCB congeners
to analyse
Proposal of index PCB
think about the list from a different perspective
- Toxicity: DL-/non-DL-PCB , endocrin disrupt potent
- Isotope labled congener : available or not
- Source estimation : homologue & congener profiles
- QA/QC : toxic congener level as impurity is very important
Proposal of index PCB
think about the list from a different perspective
- Formation/degradation mechanism :
- Complete assignment : to avoid miss assign
- Retention Time : to avoid co-elution
- Predominant congener :
- Window congener for analysis :
Index PCB congeners
should be updated,
if there’s any new information.
Present list
#1, #3, #4, #8, #9, #10, #11, #12, #15, #18, #19, #28, #31,
#33, #35, #37, #38, #44, #49, #52, #54, #57, #66, #70, #74,
#77, #78, #79, #81, #87, #95, #99, #101, #104, #105, #110,
#111, #114, #118, #123, #126, #138, #149, #153, #155, #156,
#157, #162, #167, #169, #170, #174, #178, #180, #187, #188,
#189, #194, #195, #199, #202, #203, #205, #206, #208, #209.
Sharing information
- What kind of infomation
- Where can we do
- When will we start
- Why does we share/exchange
- Who will participate
- How to share
- How to share
- Let’s start to discuss
- discuss using mailing list
Lunch session in Berlin, dioxin 2004
will be planned for similar purpose.
Acknowledgements

Matsumura for work with PCB congener
profiles.
Okuno, Tanno, Majima, Kitamoto, Gotoh,
Kurata for breast milk work.
Fujiwara, Tsurukawa for sealant work.
Fujimori for PCB destruction work.

Nakano Isamu for my life.



Thank you for your attention!
Special Thanks to Zhonie, Copper,
beautiful sky, flat corn fields
in Urbana-Champaign.
and
All participants
from
Funky Japanese guy with camera.
To All participants
from
Funky Japanese guy with camera.
You can see your pictures soon.
http://ee-net.ne.jp/illinois/
-PCB metabolite
OH-PCB, (OH)2-PCB, MeSO2-PCB
- Chiral PCBs
atropisomer; enantiomer
- Nomenclature
PCB No. [IUPAC & BZ]&Cl position
: write down both e.g. CB-94(26-235)
various PCB waste
transformer
waste sludge
soil adsorbent
capacitor
reactor
waste sludge (solid) activated carbon
PCT
stabilizer
waste cloth
PCB waste sludge（outdoor）
solidified concrete ＋asphalt pavement
Sealant containing PCB
- material that is used for sealing of building
- sealant of polysulfide polymer made during 1968-1972 contains PCB
glass window
glass window
sealant of building
joint department in floor
joint department in building
20
10
0
De10CB
40
N9CB
50
O8CB
龍野市Air
Room
air
Hp7CB
De10CB
N9CB
O8CB
Hp7CB
10
Hx6CB
10
Hx6CB
20
Pe5CB
20
Pe5CB
30
Te4CB
30
Te4CB
40
Tr3CB
40
Di2CB
60
Tr3CB
0
M1CB
50
%
50
Di2CB
30
%
De10CB
N9CB
O8CB
Hp7CB
Hx6CB
Pe5CB
Te4CB
龍野市庁舎シーリング材
Sealant
（Low-Cl)
M1CB
De10CB
N9CB
O8CB
Hp7CB
Hx6CB
Pe5CB
60
Te4CB
Tr3CB
Di2CB
M1CB
%
60
Tr3CB
Di2CB
M1CB
%
Homologue profiles of PCB in sealant and air
伊丹市庁舎シーリング材
Sealant
（High-Cl)
0
Room
air
伊丹市Air
60
50
40
30
20
10
0
PCB waste stock
transformer and capacitor : 370,000
stabilizer for Flu. lamp
: 2,400,000
non carbon paper
: 644,000 kg
waste sludge
: 117,000 kg
waste
: 105,000,000 kg
other equipments
: 33,000
waste PCB etc
:
PCB waste stock
transformer and capacitor : 370,000
stabilizer for Flu. lamp
: 2,400,000
non carbon paper
: 644,000 kg
waste sludge
: 117,000 kg
waste
: 105,000,000 kg
other equipments
: 33,000
waste PCB etc
:
PCB waste stock
transformer and capacitor : 370,000
stabilizer for Flu. lamp
: 2,400,000
non carbon paper
: 644,000 kg
waste sludge
: 117,000 kg
waste
: 105,000,000 kg
other equipments
: 33,000
waste PCB etc
:
0.4
0.35
0.3
0.25
0.2
0.15
0.1
0.05
0
#188
#188
0.3
0.2
0.1
0
#179
#176
#186
#178
#175
#187,
#182
#183
#185
#174,
#181
#177
#171
#173
#192,
#172
#180,
#193
#191
#170,
#190
#189
#179
#176
#186
#178
#175
#187,
#182
#183
#185
#174,
#181
#177
#171
#173
#192,
#172
#180,
#193
#191
#170,
#190
#189
#179
#176
#186
#178
#175
#187, #182
#183
#185
#174, #181
#177
#171
#173
#189
#180
#170, #190
#191
#180, #193
KC300-600
#174
#192, #172
#184
Mussel
#184
Sea Water
#184
#188
Intensity
0.6
0.4
0.2
0
#170
Isomer distribution (P7CB, Port Island in June,2001)
4000
KC300-600
2000
0
0.001
Sea Water
0
0.02
0.015
0.01
0.005
0
#202
#201
#199
#196 #203
#195
#205
#194
#195
#196,
#203
#199
#198
#200
#197
#201
Mussel
#202
Intensity
0.002
#194
Isomer distribution (O8CB, Port Island in June, 2001)