Transcript Science City Hydrogen Energy Seminar Advanced processes for the production of hydrogen, fuels and chemicals from waste Solid state fermentation Dan Eastwood 22nd Jan 2010 WOW Wealth.

Science City Hydrogen Energy Seminar
Advanced processes for the production of hydrogen, fuels
and chemicals from waste
Solid state fermentation
Dan Eastwood
22nd Jan 2010
WOW
Wealth Out of Waste
• Pre-treatment of plant biomass
- Low input
- Solid to liquid
• Production of chemicals
Structures found in lignin:
-aryl ether
OMe OH
OMe OH
O
O
OH
MeO
OMe
OH
OMe
RO
OAr
OR OH
MeO
CHO
O
OMe
O
OMe
O
HO 2C
OMe
OH
phenylcoumarane (12%)
OMe
OMe
OH
biphenyl (11%)
-aryl ether (51%)
OH
HO
OH
OMe
OH
OH
OH
CO 2H
HO
HO
OMe
OMe
CH 2OH
OMe
OMe
OH
O
MeO
OH
MeO
OMe
O
O
OMe
HO
diaryl propane
RO
OH
O
O
biaryl ether
biphenyl
OMe
HO
pinoresinol
phenylcoumarane
OMe
OH
pinoresinol (2%)
diarylpropane (2% )
Bioconversion Concept
MICROBIAL
BIOREFINERY
Fatty Acids &
Oils
Sugars
Aromatics
Comparison: single fermentations (brown rot fungi) &
sequential fermentations (brown rot & white rot fungi) LC-MS
Solvent soluble aromatics ~ 1.4 – 3.5 % dry wt conversion of straw
P. placenta
P. placenta followed by
P. chrysosporium
S. lacrymans
S. lacrymans followed by
P. chrysosporium
Amont of sugars released after a first fermentation with S
lacrymans and weekly incubation with cellulase
3.0
2.5
Sugars (%)
2.0
1.5
3weeks
Total s ugars
1.0
0.5
0.0
Before
-0.5
Week 1
before treat
Week 2
1 WEEK treat
Week 3
2 WEEKS treat
Treatment with cellulase
Amont of phenols released after a first fermentation with
S lacrymans and weekly incubation with cellulase
0.80
0.70
0.60
Phenoks (%)
0.50
0.40
3weeks
0.30
Total phenol s
0.20
0.10
0.00
-0.10
Before
Week 1
before treat
Week 2
1 WEEK treat
Treatment with cellulase
2 WEEKS treat
Week 3
FAME Analysis
ORGANISM
SUBSTRATE
Fatty acids
Pleurotus
ostreatus
straw
16:0, 18:2
Pleurotus
ostreatus
Straw + Nitrogen
16:0, 18:1
18:2, 20:3
Serpula
lacrymans
straw
16:0, 18:2,
20:3, 22:0
Trametes
versicolour
straw
16:0, 18:1
18:2, 20:3
22:0
Phanerochaete
chrysosporium
straw
16:0, 18:1
18:2, 20:3
Schizophillum
commune
straw
16:0, 18:1
18:2, 20:3
Warwick HRI
Chemistry
• Kerry Burton
• Andy Clark
• Kerry Kirwan
• Guy Barker
• Tim Bugg
• Claudia Koncsag
• Dave Pink
• Stuart Coles
• Arjunan Subramanian
• Alana Collis
• Mark Ahmad
WMG