Transcript Document

Figure S3, Mlynarczyk-Evans et al.
A
SS:
20%
SD:
50%
70%
30%
DD:
30%
B
navy
0 .3 5
0 .6 5
pS=
pD= (1-pS)
C
aqua
0 .5 5
0 .4 5
Calculation of proportion SD cells exhibiting a singlet signal on a given allele when chromosome states are fixed:
Proportion SD in which aqua allele is singlet = navyDaquaS/(navySaquaD + navyDaquaS)
Calculated = 0.69
Observed = 0.65 to 0.71 (Fig. 3C)
D
Model for coordination of the states of two adjacent loci along the chromosome:
pS=
navy
0 .3 5
0 .3 5
locus A
locus B
S
navy
aqua
S
AB
A
B
pS * pS
A
B
pS * pS
pD=
aqua
0 .5 5
0 .5 5
f ormulas
D
D S
AB
A B
A
B
A
B
pS * pD
pD * pS
A
B
A
B
pS * pD
pD * pS
S
navy
0 .6 5
0 .6 5
aqua
0 .4 5
0 .4 5
D
D
A B
A
B
pD * pD
A
B
pD * pD
S
S
AB
0 .1 2 2 5
0 .3 0 2 5
calculations
D
D S
AB
A B
S
0 .2 2 7 5
0 .2 4 7 5
0 .2 2 7 5
0 .2 4 7 5
D
0 .4 2 2 5
0 .2 0 2 5
Proportion concordant = [(navy ASBS * aqua ADBD) + (navy ADBD * aqua ASBS)]/[(navy ASBS * aqua ADBD) +
(navy ADBD * aqua ASBS) + (navy ASBD * aqua ADBS) + (navy ADBS * aqua ASBD)]
Predicted = 0.58
Observed = 0.64 to 0.65 (Fig. 2C)
E
Prediction of proportion SD cells exhibiting a singlet signal on a given allele in 129-tet/cas population subject to
80%/20% skewing:
Proportion SD in which 129-tet allele is singlet = 0.8(proportion aqua allele singlet) + 0.2(proportion navy allele singlet)
Predicted = 0.8(0.69) + 0.2(0.31) = 0.62
Observed = 0.60 (Fig. 5C)
D
A B