Transcript Dr. O. Tadauchi Presentation - Bee-BOL

Japanese Bee Fauna, Collections
and DNA Barcoding Project
Osamu TADAUCHI
Entomological Laboratory, Faculty of Agriculture,
Kyushu University, JAPAN
1 Database Files of Japanese and Asian
Insects
• We have constructed Entomology database
KONCHU since 1983, which is a general
database name including various files on
insects in Asia and the Pacific area
• About 165,000 records are opened via the
Internet
• One of the files, MOKUROKU is based on “A
Check List of the Japanese Insects”
Home page of an Entomology Database KONCHU
Various files in the KONCHU database
MOKUROKU
KONCHUR
DJI
ELKUType
TOBOKIBACHI
HANABACHI DB based on the Japanese bees
Result of a search: Andrena mikado
Image of various parts
Enlarged images are available
Female head
Male head
Mesoscutum
Database File TAB Based on the
Tropical Asian Bees
Example of a record: Xylocopa latipes
2 Bee Fauna in Japan 1
Bee fauna in Japan has been well investigated and revised
except for Sphecodes
The total number of Japanese bees is 420 spp. based on
our MOKUROKU file at present
Colletidae: 31spp.
Colletes: 6 spp., Hylaeus: 25 spp.
Halictidae: 120 spp.
Halictus: 4 spp., Lasioglossum 59 spp., Sphecodes: 51
spp.
Others: Nomia, Pseudapis, Lipotriches
Sphecodes needs revisional work and will decrease the
number, Lasioglossum increase it in near future
2 Bee Fauna in Japan 2
Andrenidae: 87 spp.
Andrena: 86 spp., Panurginus: 1 sp.
Melittidae: 5 spp.
Melitta, Macropis, Dasypoda
Megachilidae: 55 spp.
Megachile: 34 spp., Osmia: 7 spp., Coelioxys: 5 spp.,
Hereiades: 4 spp., etc
Apidae: 142 spp.
Nomada: 52 spp., Bombus: 22 spp., Ceratina: 10 spp.,
Xylocopa: 6 spp., Amegilla: 5 spp., Tetralonia: 5 spp., etc
2 Bee Fauna in Japan 3
We are preparing a
book on “The Bees
of Japan” including
all the Japanese
species with
various images, one
page for one species
3 Bee taxonomists and association
in Japan
1 O. Tadauchi: Andrenidae
2 Dr. S. Ikudome: Colletidae
3 Dr. R. Murao: Halictidae (Lasioglossum)
4 Dr. K. Mitai: parasitic bees (Nomada etc)
5 Mr. H. Nagase: Megachilidae
------------------------------------------------------Dr. Y. Hirashima, Dr. Y. Maeta, Dr. M. Ito
We have an association of bees in Japan (Jap. Melit.
Soc.) and have publish a newsletter once a year.
Among 70 members, about 20 persons interest in
collecting throughout in Japan
4 Bee Collections in Japan 1
1
Kyushu University, Fukuoka (100,000 indivs.)
preserved most types of bees in Japan
Japanese bee coll. (60,000 indivs., 20-30y. old)
Central Asian coll. (25,000 indivs., 5y. old)
Chinese and Korean coll. (10,000 indivs., 10y. old)
Other coll. (5,000 indvs., Bangladesh, Iran,
Indonesian, Thai and others, 1-15y. old)
We are constructing specimen database of bees
preserved in our lab. (nearly half finished), which
presented to GBIF.
4 Bee Collections in Japan 3
2 Hyogo Pref. Museum, Hyogo Pref.
Types of Tsuneki, normal Col. of Sakagami (20-50y. old)
3 Shimane University, Matsue
Japanese coll., 10-40y.old
4 Hokkaido University, Sapporo
Japanese coll., 20-70y. old
5 Private Collection: Mr. Haneda, Fukui Pref.
Japanese coll., 20-50y. old, large
6 Private Collection: Dr. Munakata, Hokkaido
Japanese coll., 20-50y. old, large
7. Others: Toyama City Museum, Toyama, Ibaragi Pref.
Museum, Mito, etc
5 DNA Barcoding Project and
Facilities
1 From April, 2008 we started to collect fresh
material for DNA barcoding. At present we
preserve about 50 spp., 400 individuals in
99% ethanol.
We will ask Japanese bee colleague to collect
fresh bee material from this year
5 DNA Barcoding Project and
Facilities
2
3
4
Two graduate students and two undergraduate
students in our lab. have started to study DNA
barcoding of bees and Cicadellidae (Hemiptera),
respectively
We used a universal primer HCO2198 as
preliminary work and could get good PCR
material
Other laboratory in the same faculty of Kyushu
University decided to help us as facilities
Preliminary test of a Japanese
Andrena used by universal primer
800bp
6 Linkage with JBOLI
1 JBOLI (organized by Prof. Ito of Tokyo
University) asked our laboratory to preserve
voucher specimen collection of Japanese
insects
2 A new species was described with
designation as the first voucher specimen in
the Japanese insects (Curculionidae,
Coleoptera) preserved in Kyushu University
(Yoshitak et al., 2008, Zootaxa)
7 Construction of a voucher
specimen database in Japan
• We will construct
voucher specimen
database of Japanese
insects
8 Funds
1
2
3
4
I have three funds related to construction of
database of Japanese insects from JSPS and JST
now (about 930,000 US$ from 2003 to 2008)
From the time being I will use the funds to
construct a database file of DNA barcoding as well
as the other database files
Associate Prof. of my laboratory succeeded in
getting a JSPS fund for DNA barcoding of
Cicadellidae (Hemiptera) from 2008 to 2010
I will apply to JSPS and other national funds such
as Ministry of Agriculture for DNA barcoding of
useful insects in the next year
9 Comments of Bees of Other
Areas in Asia 1
1
2
We made various surveys and accumulated bee
specimens in our laboratory from Korea, China,
Southeast Asia, Central Asia, Iran and Papua New
Guinea. Central Asian collection is most
representative based on five surveys by my
project in Kazakhstan and Kyrgyzstan.
I have now foreign students from Iran, Thailand
and Egypt who are studying bees. Egyptian
student started to study DNA barcoding using
Japanese Lasioglossum, Iranian studies Iranian
Andrena and Thai studies biodiversity of Thai bees.
I will instruct them about DNA barcoding and ask
to continue it after coming back from Japan.
9 Comments of Bees of Other
Areas in Asia 2
3
4
5
One of my students from China, Dr. Xu, got a job
in Chinese Agricultural University in Beijing and
is interested in DNA barcoding of bees. He will
ready to join this project.
We are now constructing TAB and TABR database
based on tropical Asian bees, which will contribute
to the study of bees in this area.
Prof. Yata of Kyushu University, who is a specialist
of butterflies, got JSPS funds and succeeded in
constructing network of entomologists and
institutes in southeast Asia. I think we can use this
network for accumulating and collecting bee
specimens.